Journal: Oncotarget

Article Title: Investigation of IL-23 (p19, p40) and IL-23R identifies nuclear expression of IL-23 p19 as a favorable prognostic factor in colorectal cancer: a retrospective multicenter study of 675 patients

doi:

Figure Lengend Snippet: A) Kaplan-Meier survival curve of test cohort showing survival time benefit of nuclear IL-23p19 positive patients particularly at early time points and B) a multivariable analysis of this cohort at 4-years follow-up. Stratification by CD8+ T-cell counts shows C) survival time benefit in patients with nuclear IL-23p19 positive cancers in CD8+ high setting with D) no benefit in CD8+ low setting.

Article Snippet: A myc-tagged IL-23p19 (IL-23A) pCMV6 expression plasmid was purchased from Origene (Labforce, Nunningen, Switzerland) and 4 μG of this plasmid were transfected into 293T HEK cells using calcium phosphate technique.

Techniques:

Journal: Oncotarget

Article Title: Investigation of IL-23 (p19, p40) and IL-23R identifies nuclear expression of IL-23 p19 as a favorable prognostic factor in colorectal cancer: a retrospective multicenter study of 675 patients

doi:

Figure Lengend Snippet: Nuclear IL-23p19 in cohort 1 stratified by CD8+ low and high groups

Article Snippet: A myc-tagged IL-23p19 (IL-23A) pCMV6 expression plasmid was purchased from Origene (Labforce, Nunningen, Switzerland) and 4 μG of this plasmid were transfected into 293T HEK cells using calcium phosphate technique.

Techniques: Mutagenesis

Journal: Oncotarget

Article Title: Investigation of IL-23 (p19, p40) and IL-23R identifies nuclear expression of IL-23 p19 as a favorable prognostic factor in colorectal cancer: a retrospective multicenter study of 675 patients

doi:

Figure Lengend Snippet: A) Kaplan-Meier survival curve of low and high CD8+ T-cell counts in patients not receiving a preoperative therapy (n=100). B) Kaplan-Meier curve showing combined nuclear IL-23p19 and CD8+ T-cell counts and effect on overall survival. Nuclear IL-23p19 positive/CD8+ high patients have a 100% survival. Effect of IL-23p19 negative/CD8+ high or IL23-p19 positive/CD8+ low has a more unfavorable intermediate effect while IL-23p19 negative/CD8+ low patients have the most limited benefit. C) Multivariable analysis showing the independent prognostic effect of the combined IL-23p19 positive/CD8+ effects on overall survival.

Article Snippet: A myc-tagged IL-23p19 (IL-23A) pCMV6 expression plasmid was purchased from Origene (Labforce, Nunningen, Switzerland) and 4 μG of this plasmid were transfected into 293T HEK cells using calcium phosphate technique.

Techniques:

Journal: Oncotarget

Article Title: Investigation of IL-23 (p19, p40) and IL-23R identifies nuclear expression of IL-23 p19 as a favorable prognostic factor in colorectal cancer: a retrospective multicenter study of 675 patients

doi:

Figure Lengend Snippet: Immunoblot showing both nuclear and cytoplasmic fractions for IL-23p19 (IL-23A) and appropriate controls.

Article Snippet: A myc-tagged IL-23p19 (IL-23A) pCMV6 expression plasmid was purchased from Origene (Labforce, Nunningen, Switzerland) and 4 μG of this plasmid were transfected into 293T HEK cells using calcium phosphate technique.

Techniques: Western Blot

Journal: Oncotarget

Article Title: Investigation of IL-23 (p19, p40) and IL-23R identifies nuclear expression of IL-23 p19 as a favorable prognostic factor in colorectal cancer: a retrospective multicenter study of 675 patients

doi:

Figure Lengend Snippet: Representative photomicrographs of positive/high (A, C, E, G) and negative/low expression (B, D, F, H) of A-B: IL-23 poly; C-D: IL-23p40; E-F: IL-23R and G -H: IL-23p19. 100 × magnification.

Article Snippet: A myc-tagged IL-23p19 (IL-23A) pCMV6 expression plasmid was purchased from Origene (Labforce, Nunningen, Switzerland) and 4 μG of this plasmid were transfected into 293T HEK cells using calcium phosphate technique.

Techniques: Expressing

Journal: Oncotarget

Article Title: Investigation of IL-23 (p19, p40) and IL-23R identifies nuclear expression of IL-23 p19 as a favorable prognostic factor in colorectal cancer: a retrospective multicenter study of 675 patients

doi:

Figure Lengend Snippet: Association of nuclear IL-23p19 expression with clinicopathological data of Cohort 1

Article Snippet: A myc-tagged IL-23p19 (IL-23A) pCMV6 expression plasmid was purchased from Origene (Labforce, Nunningen, Switzerland) and 4 μG of this plasmid were transfected into 293T HEK cells using calcium phosphate technique.

Techniques: Expressing, Mutagenesis

Journal: Scientific Reports

Article Title: Interleukin-12 and -23 blockade mitigates elastase-induced abdominal aortic aneurysm

doi: 10.1038/s41598-019-46909-y

Figure Lengend Snippet: Antagonism of IL-12p40/IL-23p19 attenuates AAA development. Mice were perfused with elastase on day 0 and injected with anti-IL-12p40 or anti-IL-23p19 or isotype control (250ug) on days 3 and 8. AD was assessed on day 14. AAA is expressed as the % increase in AD ( A , C ) or the change in AD in mm ( B , D ). Values represent mean ± SEM, n = 7–8 mice per treatment. ** p < 0.01, *** p < 0.001.

Article Snippet: For human tissue, mouse anti-human IL-12p40 (1:100 dilution; Cat# 501801, Biolegend, San Diego, CA), rabbit anti-human IL-23p19 (1:100 dilution; Cat# 3793, ProSci, Poway, CA) antibodies were employed.

Techniques: Injection, Control

Journal: Scientific Reports

Article Title: Interleukin-12 and -23 blockade mitigates elastase-induced abdominal aortic aneurysm

doi: 10.1038/s41598-019-46909-y

Figure Lengend Snippet: IL-23p19 blockade suppresses macrophage polarization. ( A ) Aortic sections obtained on day14 were double-stained for MOMA-2 (red) and IL12p40 (green) for M1 and CD206 (green) for M2. DAPI (blue) stained nuclei. Arrows indicate co-localization (yellow). Photographs are representative of 6–8 aortas per treatment. Scale bar = 25 um. ( B ) Enumeration of M1/M2 on day 14. Values represent mean ± SEM, n = 6–8 mice per treatment. ** p < 0.01, *** p < 0.001.

Article Snippet: For human tissue, mouse anti-human IL-12p40 (1:100 dilution; Cat# 501801, Biolegend, San Diego, CA), rabbit anti-human IL-23p19 (1:100 dilution; Cat# 3793, ProSci, Poway, CA) antibodies were employed.

Techniques: Staining

Journal: Scientific Reports

Article Title: Interleukin-12 and -23 blockade mitigates elastase-induced abdominal aortic aneurysm

doi: 10.1038/s41598-019-46909-y

Figure Lengend Snippet: IL-23p19 blockade suppresses MMP activity and apoptotic cell death. Aortic sections obtained on day 14 were used to detect gelatinolytic activity of MMP by in situ zymography ( A ) apoptotic cells (TUNEL + , B) , cell proliferation (PCNA + , C ). Scale bar = 50 um ( A , B ), 100 um ( C ). Values represent mean ± SEM, n = 4–6 sections per aorta, 6–8 aortas per treatment. *P < 0.05, **P < 0.001.

Article Snippet: For human tissue, mouse anti-human IL-12p40 (1:100 dilution; Cat# 501801, Biolegend, San Diego, CA), rabbit anti-human IL-23p19 (1:100 dilution; Cat# 3793, ProSci, Poway, CA) antibodies were employed.

Techniques: Activity Assay, In Situ, Zymography, TUNEL Assay

Journal: Scientific Reports

Article Title: Interleukin-12 and -23 blockade mitigates elastase-induced abdominal aortic aneurysm

doi: 10.1038/s41598-019-46909-y

Figure Lengend Snippet: IL-12/IL-23 in human AAA tissues. Immunohistochemistry of human AAA tissues revealed abundant expression of IL-12p40 and IL-23p19 (orange) throughout all layers of aortic wall tissue. Normal aortic wall tissue showed no specific staining. N = 3 independent AAA samples. L = lumen. Scale bar = 200 um.

Article Snippet: For human tissue, mouse anti-human IL-12p40 (1:100 dilution; Cat# 501801, Biolegend, San Diego, CA), rabbit anti-human IL-23p19 (1:100 dilution; Cat# 3793, ProSci, Poway, CA) antibodies were employed.

Techniques: Immunohistochemistry, Expressing, Staining